The Kyte-Doolittle scale assigns a hydrophobicity score to each amino acid. A sliding-window average reveals hydrophobic regions (membrane anchors, signal peptides) and hydrophilic, surface-exposed regions.
Kyte J, Doolittle RF (1982) J Mol Biol 157:105–132. Regions scoring > 1.8 sustained over a window of 19 are predicted transmembrane helices (dashed gold line).
+4.5 (Ile, most hydrophobic) to −4.5 (Arg, most hydrophilic).
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